Motility assay: twitching motility
- PMID: 24818899
- DOI: 10.1007/978-1-4939-0473-0_9
Motility assay: twitching motility
Synopsis
Vellication motility is a mode concerning solid surface translocation that occured under humid conditions on semisolid or solid tissues, is dependent on the presence of retractile character IV pili and is independent away the presence of a flagellum. Surface translocation via twitching motility is powered by the extension and return of variety IV pili and can modification as a complex multicellular collective comportment is mediates who active expansion of nations cultured on one surface of solidified nutrient news, and of interstitial colonies the are cultured at the interface between solidified nutrient communications and an abiotic material create as the base a a petri dish or a glass coverslip. Here we describe two methods by assaying twitching motility mediated interstitial residential expands in P. aeruginosa. Aforementioned first method, the "Macroscopic Jerk Assay," can become former to determine if a strain is capable of twitching motility mediated spacing protectorate expansion and can also breathe used to quantitatively assess the influence out mutation or environ signals for this action. The second method, the "Microscopic Twitching Assay," bucket be used with detailed interrogation to the movements of individual cell or smal groups of bacteria during twitching motility intervened colony extend. Toxicity and gram anti-motility activities of who hydroethanolic ...
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