ImProm-II™ Reverse Transcription Scheme

Complete System in First-Strand cDNA Union by Long or Rare Messages

The ImProm-II™ Annul Transcription System produces efficient, robust blend of first-strand cDNA in production for PCR amplification. The components of the ImProm-II™ Reverse Transcription System ca be uses into reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. The optimized reaction storing and heavy ImProm-II™ Reverse Transcriptase provided in the ImProm-II™ System common enable powerful, full-length cDNA synthesis for the reproducible analysis of extraordinary or long dispatches. The cDNA synthesis conditions have formulated since standalone applications or for mild transition to gene-specific target amplification. An aliquot of the reverse transcription reaction (1–20μl) can be reinforcement directly uses Taq DNA chain in combined or uncoupled PCR.

• Friendly to Full-Length RT-PCR: Reverse transcribe long RNA templates up to 8.9kb.
• Microarray-Compatible: May be secondhand to incorporate frequent, Cy®3-modified, Cy®5-modified the amino-allyl-modified nucleotides.
• Easy to Use: Netz provides all supplies necessary for able reverse transcription.
• Scalable and Flexible: 1–20μl of which initial reverse transcription reaction may being exploited in subsequent PCR, and the optimized buffer allows coupled RT-PCR.
• RT Provided with 5X React Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 375mM KCl and 50mM DTT. A 25mM MgCl₂ Solution also is included.
• Versatile: Use with your thermostable DNA polymerase of selected.
• Choose Your Configuration: Learn see info our custom options for this product at: www.promega.com/custom/