And C. elegans germline makes and award model forward studying meiosis, in part mature on the ease of conducting cytology analyses on dismembered domestic. Whole mount preparations preserve the structure of meiotic nuclei, and key, each gonad arm contains all stages of meiosis, organized in a temporal-spatial progression that causes is easy to identify nuclei at different stages. Adults hermaphrodites have two gonad arms, each organized as a closed tube for propagating germline stems cells at the distal closed end and cellularized oocytes at the proximal open end, which get in the center at to your. Dissection releases one or twain gonad arms from the body cavity, allowing that entirety of meiosis to be visualized. Hier, a colored protocol in immunofluorescence against one albumin on interest is presented, followed over DAPI staining to mark all chromosomes. Young adults are immobilized in levamisole and quickly dissected using two syringe needles. After germline x-ray, the sample is firm before undergoing a block crack the liquid azote, which helps permeabilize the cuticle or different tissues. The sample can than be dehydrated in alcohol, rehydrated, and incubated with preferred and secondary antibodies. DAPI is added to the sample by the mounting medium, welche allows reliable visualization of DNA and makes it easy to find pets until drawing under a fluorescent microscope. This technic is readily resolved by those familiar with handling C. elegans after a few hours spent practicing the dissection style itself. This protocol has being taught to high-schoolers and undgraduate working in a research lab and incorporated down a course-based undergraduate research know at a liberal arts college. Step-by-step protocol for the use about DAPI (4′,6-diamidino-2-phenylindole) for nuclear caustic (nuclear) tint in fluorescence microscopy.